Sistematik Filogenetik Isolat-isolat Kapang Indigenous Indonesia sebagai Entomopatogen Kutu Sisik (Lepidoshapes beckii Newman) Hama Tanaman Jeruk

Phylogenetic Systematics of Indonesian Indigenous Mould Isolates as Entomopathogen of Purple Scale Insect (Lepidoshapes beckii Newman) Pest of Citrus Plants

Suharjono1*, Agung P.W. Marhendra1, Anang Triwiratno2, Susi Wuryantini2, dan Lina Oktavia R1

1Jurusan Biologi, FMIPA, Universitas Brawijaya, Malang, Jl Veterang 169 Malang 65145

2Balai Penelitian Tanaman Jeruk dan Buah Subtropika (Balitjestro), Batu, Jawa Timur

  E-mail: calistus@brawijaya.ac.id    *Penulis untuk korespondensi

Abstract

Citrus fruits constitute a basic agricultural product in many countries. They are a source of national income, empowering labor market in rural zones and those surrounding urban areas, and in addition to being an important part of the diet. Insects are common problems to planting of citrus. Purple Scale, Lepidosaphes beckii (Newman) is one of the destructive insect pests of citrus worldwide. The control of purple scale insects in citrus utilizes native natural enemies including pathogens. Most known species of mould are pathogens of purple scale insect of citrus. The objective of this research was to study phylogenetic systematics of Indonesian indigenous mould isolates as pathogen on purple scale insect (Lepidoshapes beckii Newman) pest of citrus plants. Seven mould isolates derived from Purworejo, Brastagi, and Poncokusumo were showed pathogenic on purple scale insect. The fungal isolates were cultured on PDA. The DNA of fungal material was isolated with Kit Prepman Ultra (Applied Biosystems). Nuclear ribosomal Large Subunit 28S rDNA (LSU) was amplified using primers ITS1 and ITS4. Products of PCR were purified and sequenced. Based on the 28S rDNA sequence, the result was showed that isolate of PW ASG D4 was related to Gibberella moniliformis dx-12, PC ORG D4 was related to Gibberella sp. 1893, PW ASG D2 was related to Gibberella moniliformis BZ070101, PW LB D2 was related to Gibberella moniliformis 52, BRS D22 was related to Fusarium oxysporum DC-1-67, PWRJ I D4 was related to Fusarium culmorum SQ070108, and BRS D23 was related to Aschersonia sp. Ag-11.

Key words: Entomopathogen, mould, purple scale insect, phylogenetic, 28S rDNA

Abstrak

Buah jeruk merupakan produk pertanian sebagai sumber devisa, penyerap tenaga kerja, dan sebagai bahan pangan yang penting di banyak Negara. Berbagai insekta di antaranya kutu sisik coklat Lepidosaphes beckii (Newman) menjadi hama perusak pada tanaman jeruk di seluruh dunia. Pengendalian kutu sisik coklat pada tanaman jeruk dapat menggunakan kapang patogen indigenus sebagai musuh alami. Tujuan penelitian ini untuk mempelajari sistematik filogenetik tujuh isolat kapang indigenus Indonesia yang berasal dari Purworejo, Brastagi dan Poncokusumo yang patogenik pada kutu sisik coklat hama pada tanaman jeruk. Isolat-isolat kapang dibiakkan pada medium PDA dan DNA kromosom inti diisolasi dengan Kit Prepman Ultra (Applied Biosystems). Subunit besar 28S rDNA ribosom dari DNA inti diamplifikasi dengan primer ITS1 dan ITS4 kemudian amplikon dipurifikasi dan disekuensing. Berdasarkan sekuen 28S rDNA menunjukkan bahwa isolat PW ASG D4 berkerabat dengan Gibberella moniliformis dx-12, PC ORG D4 berkerabat dengan Gibberella sp. 1893, PW ASG D2 berkerabat dengan Gibberella moniliformis BZ070101, PW LB D2 berkerabat dengan Gibberella moniliformis 52, BRS D22 berkerabat dengan Fusarium oxysporum DC-1-67, PWRJ I D4 berkerabat dengan Fusarium culmorum SQ070108, dan BRS D23 berkerabat dengan Aschersonia sp. Ag-11.

Kata kunci: Kapang, entomopatogen, kutu sisik coklat, filogenetik, 28SrDNA

Biota Vol. 15 (2): 231−236, Juni 2010

ISSN 0853−8670

Fulltext/ PDF klik (Entomopatogen Kutu Sisik Jeruk_Jurnal Biota_Suharjono JB-UB)

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